Recombinant Rat Vascular Endothelial Growth Factor 164, Yeast

Product Specifications

Source

Yeast

Field of Research

Infectious Disease & Virology; Cardiovascular Research; Stem Cell & Developmental Biology

Endotoxin

Less than 0.1 EU/ug of rRtVEGF164, Yeast as determined by LAL method.

Purity

> 95 % by SDS-PAGE and 90% by SEC-HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.75-3.75 ng/ml.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Molecular Weight

Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.

Notes

For research use only.

AA Sequence

MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR