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Exonuclease I

Catalyses removal of nucleotides from single-stranded DNA in 3'-> 5' direction.

Product Specifications

Product Name Alternative

Recombinant, E. coli

Concentration

20 units/μl

Form

Liquid

Storage Conditions

Store at -20 °C. avoid freeze/thaw cycles

Notes

For research use only.

Applications Notes

Removal of residual ssDNA, including oligonucleotides, from re-action mixtures; does not degrade double-stranded DNA or RNA; requires magnesium and presence of free, accessible 3'-hydroxyl-termini; active in a wide variety of buffer conditions, allowing for direct addition of enzyme to most reaction mixtures.

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