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Recombinant Rat CD73/NT5E Protein (His Tag)

Product Specifications

Background

CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73 converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Abbreviation

CD73; NT5E

Synonyms

5'-Nucleotidase; CD73; E5NT; NT5E; Nucleotidase; ecto-5'-nucleotidase

UniProt

P21588

Accession Number

P21588

Expression System

HEK293 Cells

Tag

C-His

Sequence

Trp29-Lys549

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

59.0 kDa

Observed Molecular Weight

70-90 kDa

Formulation

2

Species

Rat

Available Sizes

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