Recombinant Vibrio cholerae Toxin B Protein

Product Specifications

Background

Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. It is responsible for the massive, watery diarrhea characteristic of cholera infection. Cholera enterotoxin subunit B (CTXB) pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.

Abbreviation

Choleragenoid; CTXB; toxB

Synonyms

Cholera enterotoxin B chain; Cholera enterotoxin gamma chain; Cholera enterotoxin subunit B; Choleragenoid; ctxB; toxB

UniProt

P01556

Accession Number

P01556

Expression System

E.coli

Tag

None

Sequence

Thr22-Asn124

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.