Recombinant Vibrio cholerae Toxin B Protein
Product Specifications
Background
Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. It is responsible for the massive, watery diarrhea characteristic of cholera infection. Cholera enterotoxin subunit B (CTXB) pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
Abbreviation
Choleragenoid; CTXB; toxB
Synonyms
Cholera enterotoxin B chain; Cholera enterotoxin gamma chain; Cholera enterotoxin subunit B; Choleragenoid; ctxB; toxB
UniProt
P01556
Accession Number
P01556
Expression System
E.coli
Tag
None
Sequence
Thr22-Asn124
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
11.8 kDa
Observed Molecular Weight
11 kDa
Species
Vibrio cholerae
Available Sizes
Documents
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