Recombinant Rhesus macaque B7-H4/VTCN1 Protein (His Tag)

Product Specifications

Background

B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins.It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.

Abbreviation

B7-H4; VTCN1

Synonyms

B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1; B7S1VCTN1; B7XPRO1291; B7h.5; B7x; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing ; Vtcn1

UniProt

F7B770

Accession Number

F7B770

Expression System

HEK293 Cells

Tag

C-His

Sequence

Phe29-Ala258

Applications

BLI

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Recombinant Rhesus Macaque B7-H4 on HIS1K Biosensor, can bind Human B7-H4 mAb with an affinity constant of 0.578 pM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.