Recombinant Klebsiella pneumoniae NEO Protein
Product Specifications
Background
Aminoglycoside 3'-phosphotransferase (APH (3') ), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH (3') is primarily found in certain species of gram-positive bacteria.
Abbreviation
NEO
Synonyms
APH (3') -II; APH (3') II; Aminoglycoside 3'-phosphotransferase; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo
UniProt
P00552
Accession Number
P00552
Expression System
E.coli
Tag
None
Sequence
Met1-Phe264
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
29 kDa
Observed Molecular Weight
26-30 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 6%Trehalose, 4%Mannitol, 0.05%Tween 80, PH8.0.
Species
Klebsiella pneumoniae
Available Sizes
More Discoveries
Explore Other Products
Browse additional items from our catalog
H5650 96 Tests
THAP domain-containing protein 3 (THAP3) Mouse ELISA Kit
Sign In for Pricing
View Details 
412714-01 100 mg
(1S,2S,3R,5S) -3-Amino-5- (2-hydroxyethoxy) cyclopentane-1,2-diol Hydrochloride
Sign In for Pricing
View Details 
412714-02 250 mg
(1S,2S,3R,5S) -3-Amino-5- (2-hydroxyethoxy) cyclopentane-1,2-diol Hydrochloride
Sign In for Pricing
View Details MBS1003260-01 1 mg (E-Coli)
Recombinant Desulfovibrio desulfuricans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial
Sign In for Pricing
View Details 
MBS1003260-02 1 mg (Yeast)
Recombinant Desulfovibrio desulfuricans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial
Sign In for Pricing
View Details