Recombinant E.coli Beta-Glucuronidase/Uida/Beta-Gus Protein (His Tag)
Product Specifications
Background
Beta-D-glucuronidase from E.coli is a highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.
Abbreviation
Uida; Beta-Gus
Synonyms
Beta-glucuronidase; GUS; uidA
UniProt
P05804
Accession Number
P05804
Expression System
E.coli
Tag
N-His
Sequence
Met1-Gln603
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
69.9 kDa
Observed Molecular Weight
72 kDa
Formulation
Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
Species
E.coli
Available Sizes
Documents
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