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Recombinant E.coli Beta-Glucuronidase/Uida/Beta-Gus Protein (His Tag)

Product Specifications

Background

Beta-D-glucuronidase from E.coli is a highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.

Abbreviation

Uida; Beta-Gus

Synonyms

Beta-glucuronidase; GUS; uidA

UniProt

P05804

Accession Number

P05804

Expression System

E.coli

Tag

N-His

Sequence

Met1-Gln603

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

69.9 kDa

Observed Molecular Weight

72 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Species

E.coli

Available Sizes

Frequently Asked Questions

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