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Recombinant Cynomolgus 5'-Nucleotidase/CD73/NT5E Protein (His Tag)

Product Specifications

Background

CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Abbreviation

CD73; NT5E

Synonyms

5'-NT;5'-Nucleotidase; CD73; Ecto-5'-Nucleotidase; NT5; NT5E; NTE

UniProt

G7P3J1

Accession Number

G7P3J1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Trp27-Lys547

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

58.8 kDa

Observed Molecular Weight

58-80 kDa

Formulation

2

Species

Cynomolgus macaques

Available Sizes

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