Recombinant Mouse TNF-alpha/TNFA Protein
Product Specifications
Background
Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.
Abbreviation
TNF-alpha; TNFA
Synonyms
Cachectin; DIF; Membrane Form; TNF-Alpha; TNF-a; TNF-alpha; TNFSF2; TNFalpha; Tnf; Tnfa; Tnfsf1a; Tnfsf2; Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2
UniProt
P06804
Accession Number
P06804
Expression System
E.coli
Tag
None
Sequence
Asp89-Leu235
Applications
Cell culture
Endotoxin
< 0.01 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 2-8 pg/ml.
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Product Datasheet
https://789.bio/ea/40tstd
Product MSDS
https://789.bio/eb/X544SK
Calculated Molecular Weight
16.4 kDa
Observed Molecular Weight
14 kDa
Species
Mouse
Available Sizes
Curated Selection
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