Recombinant Mouse RANKL/TNFSF11 Protein (His Tag)

Product Specifications

Background

Mouse tumor necrosis factor ligand superfamily member 11 (Tnfsf11) is a member of the tumor necrosis factor (TNF) cytokine family. Tnfsf11 is widely expressed in cells including T cells and T cell rich organs, such as thymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG andTNFRSF11A/RANK. Tnfsf11 is involved in a number of fundamental biological processes such as acting as regulator of interactions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cell proliferation.

Abbreviation

RANKL; TNFSF11

Synonyms

CD254; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; RANKL; Receptor activator of nuclear factor kappa-B ligand; TNF-related activation-induced cytokine; TRANCE; Tnfsf11; Tumor necrosis factor ligand superfamily member 11

UniProt

O35235

Accession Number

BAA97257.1

Expression System

HEK293 Cells

Tag

N-His

Sequence

Arg43-Asp287

Applications

BLI

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Recombinant Human OPG-Fc on Pro A Biosensor, can bind Mouse RANKL-His with an affinity constant of 1.02 pM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.