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Recombinant Mouse Carbonic Anhydrase 4/CA4 Protein (aa 17-420, His Tag)

Product Specifications

Background

Carboxypeptidase A4 (CPA4) is a member of the peptidase M14 family. CPA4 is metalloprotease that could be involved in the histone hyperacetylation pathway. CPA4 binds one zinc ion per subunit and could catalyze to release of a C-terminal amino acid, with preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val.They have distinct expression patterns and different specificities for example, preferentially cleaving aromatic (carboxypeptidase As) or basic (carboxypeptidase Bs) residues. Several, such as carboxypeptidase Xs, have lost their catalytic activity. Carboxypeptidases play important roles in digestion of food, processing of bioactive peptides and blood coagulation.

Abbreviation

Carbonic Anhydrase 4; CA4

Synonyms

CA-IV; CA4; CAIV; Car4; Carbonate dehydratase IV; EC4.2.1.1; RP17; carbonic anhydrase 4; carbonic anhydrase IVRP17; carbonic dehydratase IV; retinitis pigmentosa 17

UniProt

Q6P8K8

Accession Number

Q6P8K8

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gly17-Tyr420

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

46.7 kDa

Observed Molecular Weight

50 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.

Species

Mouse

Available Sizes

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