Recombinant Mouse Kallikrein 1/KLK1 Protein (His Tag)

Product Specifications

Background

Human tissue kallikrein (KLK1) is a serine protease, component of the KKS that has been demonstrated to exert pleiotropic beneficial effects in protection from tissue injury through its antiinflammatory, antiapoptotic, antifibrotic and antioxidative actions.Polymorphism of the human tissue kallikrein 1 (KLK1) A1789G gene is associated with susceptibility to hypertension.

Synonyms

Glandular kallikrein K1; KAL-B; Renal kallikrein; Tissue kallikrein-6; mGK-6

UniProt

P15947

Accession Number

P15947

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Asp261

Applications

Enzyme

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC) . The specific activity is > 6, 000 pmol/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.