Recombinant Mouse Tie2/CD202b Protein (aa 770-1122, His & GST Tag)

Product Specifications

Background

TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Synonyms

AA517024; Cd202b; Hyk; STK1; Tie-2; Tie2

UniProt

Q02858

Accession Number

Q02858

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Gln770-Ala1122

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 91 % as determined by reducing SDS-PAGE.

Bioactivity

1. No Kinase Activity2. Immobilized mouse TEK (aa 770-1122) at 2 μg/ml (100 μl/well) can bind human Ang2-Fc with a linear range of 0.25-2.0 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.