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Recombinant Mouse PGK1/PGKA Protein (His Tag)

Product Specifications

Background

Phosphoglycerate kinase 1 (PGK1) is an enzyme. It is mainly expressed in spermatogonia and Localized on the principle piece in the sperm. Its expression significantly decreased in the testis of elderly men. PGK1 involved in a critical energy-producing process known as glycolysis. It helps carry out a chemical reaction that converts a molecule called 1,3-diphosphoglycerate, which is produced during the breakdown of glucose, to another molecule called 3-phosphoglycerate during glycolysis. PGK1 may also act as a cofactor for polymerase alpha. The protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions.

Synonyms

Pgk-1

UniProt

P09411

Accession Number

P09411

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Val 417

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Kinase activity untested

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

46.0 kDa

Observed Molecular Weight

43 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4

Species

Mouse

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