Recombinant Human PFKFB3 (N-6His)
Product Specifications
Background
Fructose-2,6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls the activity of the enzymes phosphofructokinase 1 (PFK-1) and fructose 1,6-bisphosphatase (FBPase-1) to regulate glycolysis and gluconeogenesis. Highly phosphorylated PFKFB3 protein was found in human tumor cells, vascular endothelial cells, and smooth muscle cells. Because of its proto-oncogenic character, the PFK-2/FBPase-2 of the PFKFB3 gene is assumed to play a critical role in tumorigenesis. PFKFB3 also plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severe hypoxic conditions, which makes it a potential target for cancer therapy.
Abbreviation
PFKFB3
Synonyms
IPFK2; PFK/FBPase 3; PFK2; iPFK-2
UniProt
Q16875
Accession Number
Q16875
Expression System
E.coli
Tag
N-His
Sequence
Met1-His520
Field of Research
Signal Transduction; Cardiovascular; Neuroscience; Cancer; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
61.8 kDa
Observed Molecular Weight
57-62 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM PB, 50mM KCl, 5mM DTT, 0.1mM EDTA, 50% glycerol, 0.1% TritonX-100, pH 6.0.
Species
Human
Available Sizes
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