Recombinant Human Interleukin-33/IL-33 Protein

Interleukin-33 (IL-33) was initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules. It is a 30-32 kD pro-inflammatory protein with intracellular and extracellular activities and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is highly and selectively expressed by high endothelial venule endothelial cells (HEVECs) in human tonsils; Peyers' s patches; and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus; and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment; corresponding to mature IL-33; binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2; activates NF-kappaB and MAP kinases; and drives production of T (H) 2-associated cytokines from in vitro polarized T (H) 2 cells. In vivo; IL-33 induces the expression of IL-4; IL-5; and IL-13 and leads to severe pathological changes in mucosal organs. Human IL-33 is 270 amino acids in length.

DVS; IL1F; Interleukin; C9orf; IL-1F; IL33; C9orf26; DVS27; IL1F11; NF-HEV; NFEHEV; DVS27 related protein; IL-1F11; IL-33; Interleukin-1 family member 11; Interleukin-33; Nuclear factor from high endothelial venules; NFHEV; CHROMOSOME 9 OPEN READING FRAME 26; DKFZp586H0523; IL 1F11; IL 33; Interleukin 1 family member 11; Interleukin 33; INTERLEUKIN 33 NFHEV; Interleukin 33 precursor; Interleukin33; Interleukin-33 (109-270) ; NF HEV; Nuclear factor for high endothelial venules; OTTHUMP00000021041; RP11 575C20.2

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.