Recombinant Human CD47/IAP Protein (His Tag)

Product Specifications

Background

CD47 (Integrin-Associated Protein; IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages; neutrophils; and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin; and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution; and is reduced in expression on Rh erythrocytes.

Abbreviation

CD47; IAP

Synonyms

Antigenic Surface Determinant Protein OA3; CD47; IAP; Integrin-Associated Protein; Leukocyte Surface Antigen CD47; MER6; Protein MER6

UniProt

Q08722

Accession Number

Q08722

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gln19-Pro139

Applications

BLI

Field of Research

Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Anti-Human CD47 mAb-Fc on Protein A Biosensor, can bind Human CD47-His with an affinity constant of 0.22 nM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.