Recombinant Human LAG3/CD223 Protein (His Tag)

Product Specifications

Background

Human Lymphocyte activation gene 3 protein (LAG3) is a member of immunoglobulin (Ig) superfamily. LAG3 contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. LAG3 is involved in lymphocyte activation and can bind to HLA class-II antigens. It is selectively expressed in activated T and NK cells. LAG3 has a negative regulatory function in T cells and acts as as a new marker of T cell induced B cell activation. As a soluble molecule; LAG3 activates antigen-presenting cells through MHC class II signaling. It can lead to increased antigen-specific T-cell responses in vivo. LAG-3 has higher affinity to MHC class II than CD4.

Abbreviation

LAG3; CD223

Synonyms

CD223; LAG-3; LAG3; Lymphocyte activation gene 3 protein; Protein FDC

UniProt

P18627

Accession Number

P18627

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu23-Leu450

Applications

ELISA

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Human LAG-3-His at 5μg/ml (100 μl/well) can bind Human FGL1-mFc.The ED50 of Recombinant Human FGL1-mFc is 30-90 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.