Recombinant Human TIM-3/HAVCR2 Protein (His Tag)

Product Specifications

Background

T-Cell Membrane Protein 3 (TIM3) is a single-pass type I membrane protein that belongs to the TIM family of immunoglobulin superfamily. TIM3 includes a signal sequence (aa 1-21) ; an extracellular region (aa 22-202) with one Ig-like V-type domain; a transmembrane segment (aa 203-223) ; and a cytoplasmic domain (aa 224 - 301) . TIM3 regulates macrophage activation; inhibits T-helper type 1 lymphocyte (Th1) -mediated auto- and alloimmune responses and promotes immunological tolerance. It may be also involved in T-cell homing and as a receptor for LGALS9.

Abbreviation

TIM-3; HAVCR2

Synonyms

HAVCR2; HAVcr-2; Hepatitis A Virus Cellular Receptor 2; T-Cell Immunoglobulin and Mucin Domain-Containing Protein 3; T-Cell Membrane Protein 3; TIM-3; TIM3; TIMD-3; TIMD3

UniProt

Q8TDQ0

Accession Number

AAL65157.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ser22-Arg200

Applications

ELISA

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Anti-Human TIM-3 mAb at 2μg/ml (100 μl/well) can bind Human TIM-3-His. The ED50 of Recombinant Human TIM-3-His is 8.51ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.