Recombinant Human Interleukin-4/IL-4 Protein

Product Specifications

Background

Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation; survival and gene expression. IL-4 is produced by mast cells; T cells; and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells; characterized by their cytokine-secretion profile that includes secretion of IL-4; IL-5; IL-6; IL-10; and IL-13; which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.

Abbreviation

Interleukin-4; IL-4

Synonyms

Interleukin; B-cell growth factor; BSF; IL4; BCGF-1; BCGF1; BSF-1; BSF1; IL-4; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; Binetrakin; IGG1 induction factor; Interleukin-4; Lymphocyte stimulatory factor 1; Pitrakinra; IL2

UniProt

P05112

Accession Number

P05112

Expression System

HEK293 Cells

Tag

None

Sequence

His25-Ser153

Applications

Cell culture

Field of Research

Cancer; immunology

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.01-0.05 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.