Recombinant Human GFRRA2 Protein (His Tag)

Product Specifications

Background

Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and Neurturin, play key roles in the control of vertebrate neuronal survivial and differentiation. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGF superfamily of growth factors. Three receptors for these factors, GFRα-1, GFRα-2, and GFRα-3 have been identified. The receptors do not contain transmembrane domains and are attached to the cell membrane by glycosyl-phosphoinositol linkage. Both GFRα-1 and GFRα-2 have been shown to mediate the GDNF-dependent and Neurturin-dependent phosphorylation and activation of the tyrosine kinase Ret. GFR-3 is expressed only during development. GFRα-2 binds Neurturin and mediates activation of RET receptor tyrosine kinase by both Neurturin and GDNF.

Abbreviation

GFRRA2

Synonyms

GDNF Family Receptor Alpha-2; GDNF Receptor Alpha-2; GDNF Receptor Beta; GDNFR-Alpha-2; GDNFR-Beta; GDNFRB; GFR-Alpha-2; GFRA2; NRTNR-Alpha; NTNR-Alpha; Neurturin Receptor Alpha; RET Ligand 2; RETL2; TGF-Beta-Related Neurotrophic Factor Receptor 2; TRNR2

Accession Number

O00451

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ser22-Ser441

Field of Research

Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.