Recombinant Human CD3 ε/CD3E Protein (His Tag)
Product Specifications
Background
T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) . TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Abbreviation
CD3 ε; CD3E
Synonyms
CD3 epsilon; CD3E; CD3e; IMD18; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; T-Cell Surface Glycoprotein CD3 Epsilon Chain; T3E
UniProt
P07766
Accession Number
P07766
Expression System
HEK293 Cells
Tag
C-His
Sequence
Asp23-Asp126
Applications
ELISA
Field of Research
Immunology; Stem cells
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Immobilized Human CD3E-His at 10 ug/ml (100 ul/wel) can bind Human Anti-CD3 mAb. The ED50 of Recombinant Human Anti-CD3 mAb is 3-15 ng/ml.
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Product Datasheet
https://789.bio/ea/iushpo
Product MSDS
https://789.bio/eb/mHGmn1
Calculated Molecular Weight
12.8 kDa
Observed Molecular Weight
15-21 kDa
Species
Human
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
