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Recombinant Human RPE Protein (E.coli, His Tag)

Product Specifications

Background

Ribulose-Phosphate 3-Epimerase (RPE) is a member of the Ribulose-Phosphate 3-Epimerase family. RPE exists as a homodimer and catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate. RPE binds one divalent metal cation per subunit and contains tightly bound Fe2+ when produced in E. coli, but the physiological cofactor may be Co2+, Mn2+ or Zn2+. It has been shown that RPE participates in 3 metabolic pathways: pentose phosphate pathway, pentose and glucuronate interconversions, and carbon fixation.

Abbreviation

RPE

Synonyms

HUSSY-17; RPE; RPE2-1; Ribulose-5-Phosphate-3-Epimerase; Ribulose-Phosphate 3-Epimerase

UniProt

Q96AT9

Accession Number

Q96AT9-1

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Arg228

Field of Research

Signal Transduction, Cancer, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

25.9 kDa

Observed Molecular Weight

28 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 6.2.

Species

Human

Available Sizes

Frequently Asked Questions

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