Recombinant Human UBE2T Protein (His Tag)
Product Specifications
Background
Ubiquitin-Conjugating Enzyme E2 T (UBE2T) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2T accepts the ATP-dependent ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro; UBE2T is able to catalyze polyubiquitination using all 7 ubiquitin Lys residues; but may prefer 'Lys-11'-; 'Lys-27'-; 'Lys-48'- and 'Lys-63'-linked polyubiquitination. UBE2T is an important factor of the Faconi anemia pathway of DNA damage repair and; upon self-inactivation; may negatively regulate the Faconi pathway.
Abbreviation
UBE2T
Synonyms
Cell Proliferation-Inducing Gene 50 Protein; UBE2T; Ubiquitin Carrier Protein T; Ubiquitin-Conjugating Enzyme E2 T; Ubiquitin-Protein Ligase T
UniProt
Q9NPD8
Accession Number
Q9NPD8
Expression System
E.coli
Tag
N-His
Sequence
Met 1-Val197
Field of Research
Cell biology; epigenetics and nuclear signal
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
24.7 kDa
Observed Molecular Weight
25-30 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
Species
Human
Available Sizes
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