Recombinant Human CSF2RA/GM-CSFR Protein (His Tag)

Product Specifications

Background

Granulocyte-Macrophage Colony-Stimulating Factor Receptor Subunit α (CSF2RA) is a single-pass type I membrane protein which belongs to the type I cytokine receptor family of Type 5 subfamily. The CSF2RA gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes with some of the isoforms being membrane-bound and others being soluble. CSF2RA is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CSF2RA transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. Defects in CSF2RA are the cause of pulmonary surfactant metabolism dysfunction type 4 (SMDP4) .

Abbreviation

CSF2RA; GM-CSFR

Synonyms

CD116; CDw116; CSF2R; CSF2RA; CSF2RY; GM-CSF-R-Alpha; GMCSFR-Alpha; GMR-Alpha; Granulocyte-Macrophage Colony-Stimulating Factor Receptor Subunit Alpha

UniProt

P15509

Accession Number

P15509

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu23-Gly320

Applications

Cell culture

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit GM-CSF-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.5-2 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.