Recombinant Human UBA5 Protein (His Tag)
Product Specifications
Background
UBA5 is a member of the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are recognized as covalently conjugated to various cellular substrates by a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) has a vital role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. UBA5 is located primarily in cytoplasm, while it generally localizes to the nucleus in presence of SUMO2.
Abbreviation
UBA5
Synonyms
ThiFP1; UBA5; UBE1DC1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1; Ubiquitin-like modifier-activating enzyme 5
UniProt
Q9GZZ9
Accession Number
Q9GZZ9
Expression System
E.coli
Tag
N-His
Sequence
Met 1-Met404
Field of Research
Cell biology
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 90 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
47.0 kDa
Observed Molecular Weight
14&40-60 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 50mM NaCl, 1mM DTT, 10% Glycerol, pH8.0.
Species
Human
Available Sizes
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