Recombinant Human UCHL3/UCH-L3 Protein (His Tag)
Product Specifications
Background
Ubiquitin Carboxyl-Terminal Hydrolases (UCHs) are a family of cysteine hydrolases. They catalyze the hydrolysis of amides, thioesters and esters, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin. Up regulation of UCHL3 is associated with uterine cervical neoplasms. UCHL3 is implicated in age related cognitive disorders. UCHL3 also promotes adipogenesis and insulin signaling. In mice, UCHL3 knockout have been shown to be resistant to diet-induced obesity.
Abbreviation
UCHL3; UCH-L3
Synonyms
UCH-L3; UCHL3; Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3; Ubiquitin Thioesterase L3
UniProt
P15374
Accession Number
P15374
Expression System
E.coli
Tag
C-His
Sequence
Met 1-Ala230
Field of Research
Cell biology; epigenetics and nuclear signal
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
27.3 kDa
Observed Molecular Weight
25 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.0.
Species
Human
Available Sizes
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