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Recombinant Human Thymidylate Kinase/DTYMK Protein (His Tag)

Product Specifications

Background

Thymidylate kinase (DTYMK) is a ubiquitous enzyme of about 25 kD which belongs to thymidylate kinase family. DTYMK is important in the dTTP synthesis pathway for DNA synthesis. It participated in the pyrimidine metabolism pathway and dTTP biosynthesis pathway. DTYMK catalyzes the conversion of dTMP to dTDP and catalyzes the phosphorylation of thymidine 5'-monophosphate (dTMP) to form thymidine 5'-diphosphate (dTDP) in the presence of ATP and magnesium. Structural and functional analyses suggest that the cDNA codes for authentic human dTMP kinase. The mRNA levels and enzyme activities corresponded to cell cycle progression and cell growth stages.

Abbreviation

Thymidylate Kinase; DTYMK

Synonyms

CDC8; DTYMK; TMPK; TYMK; Thymidylate kinase; dTMP kinase

UniProt

P23919

Accession Number

P23919

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Lys212

Field of Research

Epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Product Datasheet

https://789.bio/ea/hvyask

Product MSDS

https://789.bio/eb/D8GmjD

Calculated Molecular Weight

24.9 kDa

Observed Molecular Weight

25-31 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Sucrose, 0.05% Tween 80, 20% Glycerol, 1mM EDTA, pH8.0.

Species

Human

Available Sizes

Curated Selection

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