Recombinant Human SCF/c-Kit Ligand Protein (His Tag)

Product Specifications

Background

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid; erythroid; and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.

Abbreviation

SCF; c-Kit Ligand

Synonyms

KITLG; Kit Ligand; MGF; Mast Cell Growth Factor; SCF; Stem Cell Factor; c-Kit ligand

UniProt

P21583

Accession Number

P21583

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu26-His214

Applications

Cell culture

Field of Research

Developmental Biology; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 2-10 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.