Recombinant Human SCF/c-Kit Ligand Protein (His Tag)
Product Specifications
Background
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid; erythroid; and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
Abbreviation
SCF; c-Kit Ligand
Synonyms
KITLG; Kit Ligand; MGF; Mast Cell Growth Factor; SCF; Stem Cell Factor; c-Kit ligand
UniProt
P21583
Accession Number
P21583
Expression System
HEK293 Cells
Tag
C-His
Sequence
Glu26-His214
Applications
Cell culture
Field of Research
Developmental Biology; immunology; Stem cells
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 2-10 ng/ml.
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
22.0 kDa
Observed Molecular Weight
30-50 kDa
Species
Human
Available Sizes
Documents
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