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Recombinant Human Ribonuclease A/RNASE1 Protein (His Tag)

Product Specifications

Background

Ribonuclease Pancreatic is a secreted enzyme that belongs to the pancreatic ribonuclease family. RNASE1 is an endonuclease that cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. RNASE1 prefers poly (C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNASE1 acts on single stranded and double stranded RNA.

Abbreviation

Ribonuclease A; RNASE1

Synonyms

HP-Rnase; RIB-1; RIB1; RNASE1; RNS1; RNase 1; RNase A; RNase UpI-1; Ribonuclease 1; Ribonuclease A; Ribonuclease Pancreatic

UniProt

P07998

Accession Number

P07998

Expression System

HEK293 Cells

Tag

C-His

Sequence

Lys29-Thr156

Field of Research

Epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

15.6 kDa

Observed Molecular Weight

17-33 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Glycerol, pH 7.4.

Species

Human

Available Sizes

Frequently Asked Questions

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