Recombinant Human PKLR Protein (His Tag)

Product Specifications

Background

Pyruvate Kinase Isozymes R/L (PKLR) belongs to the pyruvate kinase family, There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver; R is found in red cells; M1 is the main form in muscle, heart and brain; M2 is found in early fetal tissues. PKLR exists as a homotetramer and catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in PKLR are also the cause of pyruvate kinase deficiency of red cells, which is a frequent cause of hereditary non-spherocytic hemolytic anemia.

Abbreviation

PKLR

Synonyms

PK1; PKL; PKLR; Pyruvate Kinase 1; Pyruvate Kinase Isozymes R/L; R-Type/L-Type Pyruvate Kinase; Red Cell/Liver Pyruvate Kinase

UniProt

P30613

Accession Number

P30613

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ser574

Field of Research

Signal Transduction; Cardiovascular; Cancer; epigenetics and nuclear signal; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

62.9 kDa

Observed Molecular Weight

58 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 5% Trehalose, 5% Mannitol, 0.02% Tween 80, 50% Glycerol, 1mM EDTA, 1mM DTT, pH8.0.