Recombinant Human PPP1R14A Protein (His Tag)
Product Specifications
Background
Protein Phosphatase 1 Regulatory Subunit 14A (PPP1R14A) belongs to the PP1 inhibitor family. PPP1R14A is mapped to chromosome 19q13.13-q13.2. PPP1R14A binds directly to protein kinase C and casein kinase I. Meantime; PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. PPP1R14A is the inhibitor of PPP1CA. When phosphorylated; PPP1R14A has over 1000-fold higher inhibitory activity; creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. In addition; inhibition of PPP1R14A also enhances contraction of smooth muscle in the absence of increment of intracellular Ca2+ concentration.
Abbreviation
PPP1R14A
Synonyms
17 kDa PKC-Potentiated Inhibitory Protein of PP1; CPI-17; CPI17; PPP1INL; PPP1R14A; Protein Kinase C-Potentiated Inhibitor Protein of 17 kDa; Protein Phosphatase 1 Regulatory Subunit 14A
UniProt
Q96A00
Accession Number
Q96A00
Expression System
E.coli
Tag
C-His
Sequence
Met 1-Pro147
Field of Research
Signal Transduction
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
17.8 kDa
Observed Molecular Weight
23-26 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 0.2mM EDTA, 1mM DTT, 10% Glycerol, pH 8.0.
Species
Human
Available Sizes
Documents
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