Recombinant Human PCSK9 Protein (D374Y, His Tag)
Product Specifications
Background
Recombinant Human Proprotein Convertase Subtilisin/Kexin Type 9/PCSK9 (D374Y) is a gain of function mutant of human PCSK9 protein. Human PCSK9 is a secretory subtilase belonging to the proteinase K subfamily. PCSK9 is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the ER, the pro domain and mature chain are secreted together through noncovalent interactions. PCSK9 binds with low-density lipoprotein receptor (LDLR) and it plays a major regulatory role in cholesterol homeostasis. Inhibition of PCSK9 function by preventing PCSK9/LDLR interaction is currently being explored as a means of lowering cholesterol levels. PCSK9 also binds to apolipoprotein receptor 2 (ApoER2), and play a role in the neural development.
Abbreviation
PCSK9
Synonyms
NARC-1; NARC1; Neural Apoptosis-Regulated Convertase 1; PC9; PCSK9; Proprotein Convertase 9; Proprotein Convertase Subtilisin/Kexin Type 9; Subtilisin/Kexin-Like Protease PC9
UniProt
Q8NBP7
Accession Number
Q8NBP7
Expression System
HEK293 Cells
Tag
C-His
Sequence
Gln31-Gln692 (Asp374Tyr, Val474Ile, Gly504Arg, Gly670Glu)
Applications
ELISA
Field of Research
Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism; Stem cells
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 90 % as determined by reducing SDS-PAGE.
Bioactivity
50
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
15-18&60-80 kDa
Observed Molecular Weight
13.8&58.2 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 20% Glycerol, pH 7.4.
Species
Human
Available Sizes
Documents
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