Recombinant Human PCSK9 Protein (AVI Tag)

Product Specifications

Background

Human Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) is a secretory subtilase belonging to the proteinase K subfamily. PCSK9 is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the ER ; the pro domain and mature chain secrete together through noncovalent interactions. PCSK9 binds with low-density lipoprotein receptor (LDLR) and plays a major regulatory role in cholesterol homeostasis. Inhibition of PCSK9 function by preventing PCSK9/LDLR interaction is currently being explored as a means of lowering cholesterol levels. PCSK9 also binds to apolipoprotein receptor 2 (ApoER2) ; and play a role in the neural development.

Abbreviation

PCSK9

Synonyms

NARC-1; NARC1; Neural Apoptosis-Regulated Convertase 1; PC9; PCSK9; Proprotein Convertase 9; Proprotein Convertase Subtilisin/Kexin Type 9; Subtilisin/Kexin-Like Protease PC9

UniProt

Q8NBP7

Accession Number

Q8NBP7

Expression System

HEK293 Cells

Conjugation

Biotinylated

Tag

C-Avi

Sequence

Gln31-Gln692 (Val474Ile, Gly670Glu)

Applications

ELISA

Field of Research

Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Human LDLR-His (Cat: PKSH033435) at 10μg/ml (100 μl/well) can bind Human PCSK9-BiotinylatedAVI. The ED50 of Human PCSK9-BiotinylatedAVI is 126.41ug/ml.