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Recombinant Human HMBS Protein (His Tag)

Product Specifications

Background

Porphobilinogen Deaminase (HMBS) is a member of the HMBS family. PBGD is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. HMBS is involved in the production of heme, which is important for all of the body's organs, although it is most abundant in the blood, bone marrow, and liver. In addition, Heme is an essential component of iron-containing proteins called hemoproteins, including hemoglobin. Defects in PBGD are the cause of acute intermittent porphyria.

Abbreviation

HMBS

Synonyms

HMBS; Hydroxymethylbilane Synthase; PBG-D; PBGD; Porphobilinogen Deaminase; Pre-Uroporphyrinogen Synthase; UPS

UniProt

P08397

Accession Number

P08397

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ser2-His361

Field of Research

Signal Transduction; Cell biology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

40.5 kDa

Observed Molecular Weight

47 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 5% mannitol, 50% Glycerol, 0.1% Tween 80, pH7.4.

Species

Human

Available Sizes

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