Recombinant Human POMGNT1 Protein (His Tag)
Product Specifications
Background
Protein O-Linked-Mannose β-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3) .
Abbreviation
POMGNT1
Synonyms
2-N-Acetylglucosaminyltransferase 1;2-N-Acetylglucosaminyltransferase I.2; MGAT1.2; POMGNT1; POMGnT1; Protein O-Linked-Mannose Beta-1; UDP-GlcNAc:Alpha-D-Mannoside Beta-1
UniProt
Q8WZA1
Accession Number
Q8WZA1
Expression System
HEK293 Cells
Tag
C-His
Sequence
Leu59-Thr660
Field of Research
Tags & Cell Markers
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 90 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
69.3 kDa
Observed Molecular Weight
74 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.
Species
Human
Available Sizes
Documents
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