Recombinant Human PTH1R Protein (Gly49, His Tag)

Product Specifications

Background

Parathyroid hormone 1 receptor (PTH1R) is a multi-pass membrane protein. The protein is expressed in high levels in bone and kidney and regulates calcium ionhomeostasis through activation of adenylate cyclase and phospholipase C. In bone, it is expressed on the surface of osteoblasts. When the receptor is activated through PTH binding, osteoblasts express RANKL (Receptor Activator of Nuclear Factor kB Ligand), which binds to RANK (Receptor Activator of Nuclear Factor kB) on osteoclasts. This turns on osteoclasts to ultimately increase the resorption rate.

Abbreviation

PTH1R

Synonyms

PTH/PTHr receptor; PTH/PTHrP type I receptor; PTH1 receptor; PTH1R; Parathyroid hormone 1 receptor; Parathyroid hormone/parathyroid hormone-related peptide receptor

UniProt

Q0VGD7

Accession Number

Q0VGD7

Expression System

HEK293 Cells

Tag

C-His

Sequence

Tyr23-Met 189

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.