Recombinant Human NAD Kinase/NADK Protein (His Tag)
Product Specifications
Background
NAD Kinase (NADK) is an enzyme that belongs to the NAD Kinase family. It is a widely expressed enzyme, but it is not detected in skeletal muscle. NADK converts Nicotinamide Adenine Dinucleotide (NAD+) into NADP+, through phosphorylating the NAD+ coenzyme. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. The structure of the NADK from the archaean Archaeoglobus fulgidus has been determined.
Abbreviation
NAD Kinase; NADK
Synonyms
NAD Kinase; NADK; Poly (P) /ATP NAD Kinase
UniProt
O95544
Accession Number
AAH01709.1
Expression System
E.coli
Tag
N-His
Sequence
Ser64-Gly446
Field of Research
Signal Transduction; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
44.4 kDa
Observed Molecular Weight
49 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, pH 7.5.
Species
Human
Available Sizes
Documents
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