Recombinant Human SMAD3 Protein (His & Flag Tag)
Product Specifications
Background
Mothers against decapentaplegic homolog 3 (SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.
Abbreviation
SMAD3
Synonyms
JV15-2; MAD homolog 3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; SMAD family member 3; SMAD3; Smad3; hMAD-3; hSMAD3
UniProt
P84022
Accession Number
P84022
Expression System
E.coli
Tag
N-His-Flag
Sequence
Ser2-Ser425
Field of Research
Signal Transduction; Cancer; epigenetics and nuclear signal; metabolism; Stem cells
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 85 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
50.5 kDa
Observed Molecular Weight
50-60 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 10% Glycerol, 2mM EDTA, pH 8.0.
Species
Human
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
TA505600BM 100 µL
TRAPPC4 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1A3]
Ask
View Details