Recombinant Human MICA Protein (Fc Tag)

Product Specifications

Background

MHC class I polypeptide-related sequence A, also known as MIC-A, PERB11.1 and MICA, is a single-pass type I membrane protein which belongs to the MHC class I family of MIC subfamily. MICA contains one Ig-like C1-type domain and is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA is the ligand for NK cell activating receptor KLRK1/NKG2D. MICA seems to have no role in antigen presentation. MICA leads to cell lysis by binding to KLRK1.

Abbreviation

MICA

Synonyms

MHC Class I Polypeptide-Related Sequence A; MIC-A; MICA; PERB11.1

UniProt

Q29983

Accession Number

AAH16929.1

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Glu24-Gln308

Applications

ELISA

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Mouse NKG2D at 2 μg/ml (100 μl/well) can bind Human MICA (C-Fc) . The EC50 of Human MICA (C-Fc) is ≤10 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.