Recombinant Human Legumain/LGMN Protein (His Tag)
Product Specifications
Background
Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.
Abbreviation
Legumain; LGMN
Synonyms
Asparaginyl Endopeptidase; LGMN; Legumain; PRSC1; Protease Cysteine 1
UniProt
Q99538
Accession Number
AAH03061.1
Expression System
HEK293 Cells
Tag
C-His
Sequence
Ile18-Tyr433
Field of Research
Signal Transduction; Cell biology; Cancer; immunology; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
48.7 kDa
Observed Molecular Weight
56 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.
Species
Human
Available Sizes
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