Recombinant Human IPPI2/IDI2 Protein (His Tag)

Product Specifications

Background

Isopentenyl Pyrophosphate Isomerase 2 (IDI2) belongs to the IPP isomerase type 1 family. Both isozymes, IDI1 and IDI2 are localized to the peroxisome by a PTS1-dependent pathway. IDI2 is expressed in skeletal muscle, which contains one nudix hydrolase domain. IDI2 binds one magnesium per subunit. IDI2 catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP) . It is reported that IDI2 is regulated independently from IDI1, by a mechanism that may involve PPAR-α.

Abbreviation

IPPI2; IDI2

Synonyms

IDI2; IPP Isomerase 2; IPPI2; Isopentenyl Pyrophosphate Isomerase 2; Isopentenyl-Diphosphate Delta-Isomerase 2

UniProt

Q9BXS1

Accession Number

Q9BXS1

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Val227

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

28.9 kDa

Observed Molecular Weight

28-31 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT, 0.1mM PMSF, pH 8.0.

Species

Human

Available Sizes

Documents

Product Datasheet

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