Recombinant Human IDH1 Protein (R132H, C-8His)
Product Specifications
Background
Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 exists as a homodimer; binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma. IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions; such as the conversion of 2; 4-dienoyl-CoAs to 3-enoyl-CoAs; as well as in peroxisomal reactions that consume 2-oxoglutarate; namely the α-hydroxylation of phytanic acid.
Abbreviation
IDH1
Synonyms
Cytosolic NADP-Isocitrate Dehydrogenase; IDH; IDH1; IDP; Isocitrate Dehydrogenase [NADP] Cytoplasmic; NADP (+) -Specific ICDH; Oxalosuccinate Decarboxylase; PICD
UniProt
O75874
Accession Number
O75874
Expression System
E.coli
Tag
C-His
Sequence
Met1-Leu414 (Arg132His)
Field of Research
Signal Transduction; Cancer; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
48.1 kDa
Observed Molecular Weight
40-50 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 4% Sucrose, 50% glycerol, 0.02% Tween 80, pH 8.0.
Species
Human
Available Sizes
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