Recombinant Human Interleukin-7/IL-7 Protein (His Tag)

Product Specifications

Background

Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65% sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT; which regulate lymphocyte survival; glucose uptake; proliferation; and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.

Abbreviation

Interleukin-7; IL-7

Synonyms

Lymphopoietin; IL7; IL-7; IL 7; Interleukin 7; Interleukin-7; LP-1; Lymphopoietin 1

UniProt

P13232

Accession Number

P13232

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asp26-His177

Applications

Cell culture

Field of Research

Immunology

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL) . The ED50 for this effect is 50-300 pg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.