Recombinant Human Interleukin-3/IL-3 Protein (Human Cells, His Tag)

Product Specifications

Background

Interleukin-3 (IL-3) is a potent growth promoting cytokine. IL-3 can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological function through binding to specific cell surface receptors. The amino acid sequences of this protein among different species share relatively low identity and its activity is highly species-specific. IL-3 has also been shown to possess neurotrophic activity; and is thought to be associated with neurologic disorders.

Abbreviation

Interleukin-3; IL-3

Synonyms

MLN; Interleukin; S100A; IL3; IL-3; MCGF; MULTI-CSF; Hematopoietic Growth Factor; Interleukin-3; Mast Cell Growth Factor; MCSF; Multipotential Colony-Stimulating Factor; P-Cell Stimulating Factor; P-Cell-Stimulating Factor; S100A11; MLN70; S100C

UniProt

P08700

Accession Number

P08700

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala20-Phe152

Applications

Cell culture

Field of Research

Immunology

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.3-1. 5 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.