Recombinant Human IL2Ra/CD25 Protein (Fc Tag)

Product Specifications

Background

Interleukin-2 receptor subunit alpha (IL2RA) is a single-pass type I membrane protein; contains 2 Sushi (CCP/SCR) domains. The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains; together with the common gamma chain (IL2RG) ; constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor; while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein; soluble IL2RA has been isolated and determined to result from extracellular proteolyisis.

Abbreviation

IL2Ra; CD25

Synonyms

IMD; IDDM; IL2RA; CD25; IDDM10; IL2R; IMD41; TCGFR; p55; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2-RA; IL-2-RA; IL-2Rα; Interleukin-2 receptor subunit alpha; TAC antigen; Tac; p55; IL-2Rα alpha chain; CD25 antigen; IL 2 receptor alpha subunit; IL2 RA; Interleukin 2 receptor; Interleukin 2 receptor alpha; Interleukin 2 receptor alpha chain; t-cell growth factor receptor

UniProt

P01589

Accession Number

P01589

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Glu22-Cys213

Applications

BLI

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Human IL-2-His on HIS1K Biosensor, can bind Human IL-2RA-Fc with an affinity constant of 3.06 nM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.