Recombinant Human HGPRT/HPRT1 Protein (His Tag)
Product Specifications
Background
Hypoxanthine-Guanine Phosphoribosyltransferase (HGPRT) has an important role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 functions to salvage purines from degraded DNA to renewed purine synthesis, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP.
Abbreviation
HGPRT; HPRT1
Synonyms
HGPRT; HGPRTase; HPRT; HPRT1; Hypoxanthine-Guanine Phosphoribosyltransferase
UniProt
P00492
Accession Number
P00492
Expression System
E.coli
Tag
N-His
Sequence
Met 1-Ala218
Field of Research
Cell biology; epigenetics and nuclear signal
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 85 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
27.8 kDa
Observed Molecular Weight
29 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, 50% Glycerol, pH8.0.
Species
Human
Available Sizes
Documents
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