Recombinant Human GRB2 Protein (His Tag)
Product Specifications
Background
As an adaptor protein, Growth Factor Receptor-Bound Protein 2 (GRB2) is involved in siganl transduction and consists of a central SH2 domain flanked by two SH3 domains. GRB2 associates with activated Tyr-phosphorylated EGF receptor/EGFR and PDGF receptors via its SH2 domain, stimulating GTP binding to Ras, which in turn activates MAPK and other signaling pathway.It also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK. probably via the concerted action of both its SH2 and SH3 domains.
Abbreviation
GRB2
Synonyms
ASH; Adapter Protein GRB2; GRB2; Growth Factor Receptor-Bound Protein 2; Protein Ash; SH2/SH3 Adapter GRB2
UniProt
P62993
Accession Number
P62993
Expression System
E.coli
Tag
C-His
Sequence
Met 1-Val217
Field of Research
Signal Transduction; Cancer
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 90 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
26.3 kDa
Observed Molecular Weight
25-30 kDa
Species
Human
Available Sizes
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